spoVFB [2019-08-01 11:21:08]

dipicolinate synthase (subunit B)

locus: BSU_16740

pI: 6.15

mw: 21.72 kDa

protein length: 200 aa

gene length: 603 bp

function: dipicolic acid production

product: dipicolinate synthase (subunit B)

essential: no

synonyms: dpaB

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.6|Miscellaneous metabolic pathways] → [category|SW 2.6.6.3|Biosynthesis of dipicolinate]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]

Gene

Coordinates: 1,745,263 1,745,865

The protein

Structure

• [PDB|3MCU] (from B. cereus, 72% identity)

Expression and Regulation

Operons

• genes: [gene|3DA80060554B78E9DC2189CECB78680095B08B32|spoVFA]-[gene|4D93A13DB9DC55A49525BFF4FD589F4FF67D50E8|spoVFB]-[gene|564A2F43F8AABE97FC8F0B1B3382A9F31F02FA18|asd]-[gene|6D6718AC72C9D86EDE16B191EDAB3ABA488B689B|dapG]-[gene|00B4CA4E25858A56F9D4E0ADEE2643F203FEC613|dapA]
  description: [Pubmed|8098035]

  sigma factors

  • [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK]: sigma factor, [Pubmed|15699190,8098035], in [regulon|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK regulon]

  regulation

  • expressed late during sporulation in the mother cell ([protein|search|SigK]) [Pubmed|15699190,8098035]

Biological materials

Mutant

• BKE16740 ([gene|4D93A13DB9DC55A49525BFF4FD589F4FF67D50E8|spoVFB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE16740 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTTCCTTTTAATGACGACA, downstream forward: _UP4_TAAATTTACAGAAAATCTTT
• BKK16740 ([gene|4D93A13DB9DC55A49525BFF4FD589F4FF67D50E8|spoVFB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK16740 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTTCCTTTTAATGACGACA, downstream forward: _UP4_TAAATTTACAGAAAATCTTT

References

• 8345520,8098035,15699190